Home|Journals|Articles by Year|Audio Abstracts

Original Article

J App Pharm Sci. 2022; 12(12): 84-94

Proapoptotic activity of essential oils from Syzygium aromaticum, Melaleuca cajuputi, and Cymbopogon nardus on HeLa human cervical cancer cells

Happy Kurnia Permatasari, Anita Dominique Subali, Muhammad Yusuf, Holipah Holipah.

Cited by 1 Articles

Cancer develops through an imbalance in the cell cycle regulators, resulting in persistent proliferation and escaping apoptosis. Essential oils (EOs) are gaining popularity as a potential natural-based anticancer agent. This research aimed to evaluate cytotoxic activity by induction of apoptosis in Syzygium aromaticum, Melaleuca cajuputi, and Cymbopogon nardus EOs against the HeLa human cervical cancer cell lines. HeLa cell cultures were treated with EOs from three plants separately at 30, 60, and 120 μg/ml and 0 μg/ml as control. The trypan blue exclusion test was conducted to examine cell viability and determine the inhibitory concentration (IC50) value. Cytotoxicity activity was evaluated using Annexin V (AV) and propidium iodide (PI) flow cytometry. The EOs of the three herbs significantly decrease HeLa cells’ viability at 24 and 48 hours after treatment in comparison to the control. AV–PI analysis displayed significant induction of late-stage apoptosis in a dose-dependent relationship. The 48 hour IC50 values of EOs were 18.06 μg/ml (S. aromaticum), 26.25 μg/ml (M. cajuputi), and 28.03 μg/ml (C. nardus). The EOs of S. aromaticum, M. cajuputi, and C. nardus have potential anticancer activity with their cytotoxic effects on HeLa cells and could be explored further as a cervical cancer treatment.

Key words: anti-cancer, apoptosis, cervical cancer, cytotoxic, essential oils, HeLa cell

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.