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Chemotherapeutic effects of doxorubicin loaded PEG coated TiO nanocarriers on breast cancer cell lines

Ayca Tas, Nese Kekliklicioglu Cakmak, Erkan Gumus, Mustafa Atabey, Yavuz Silig.

Abstract
Aim: Breast cancer is the most common frequently diagnosed malignancy among women and leading cause of cancer death in women worldwide. The aim of this study is 1) to increase the biocompatibility of the titanium dioxide (TiO) nanoparticles (NPs) by coating it with PolyEthylene Glycol (PEG) and to develop a new nanostructure system and 2) to determine anticancer activity of doxorubicin (DOX) loaded PEG-TiO on MDA-MB-231 cell lines.
Material and Methods: TiO nanoparticles used in this study were synthesized, coated with PEG, and PEG-TiO nanostructure system was loaded by DOX. UV analysis was performed on the prepared solutions. The synthesized drugs were applied to the MDA-MB-231 breast cancer cell line and cytotoxic effect of these drugs were determined by using MTT method. The MDA-MB-231 cells were treated with different concentrations of TiO (5-100 μg/ml) for 24, 48 and 72 hours. Apoptosis and necrosis were determined by fluorescence microscopy using the Hoechst 33258 (HO) /propidium iodide (PI) double staining.
Results: The effects of TiO, PEG-TiO, DOX, and TiO-PEG-DOX on the MDA-MB-231 cell line were compared with the control group and IC50 values were determined for 24, 48 and 72 hours.
Conclusion: In this study, it was shown that the effect of TiO-PEG-DOX nanostructured system on MDA-MB-231 cell line was inhibition growth in cancer cells and induction of apoptosis when compared with control group and DOX.

Key words: Breast Cancer; MDA-MB-231; TiO2-PEG-DOX: DOX.


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