In Vitro Development Competence of Buffalo Oocytes- Effect of Oocytes Quality on Maturation, Embryonic Developments and Apoptosis
Lalit Mohan Jeena, Dharmendra Kumar, Sandeep Rahangdale, Ajit Pratap Singh, Bikash Chandra Sarkhel.
The present study undertaken to assess the effect of different grades of cumulus-oocyte complexes (COCs) on in vitro maturation and embryonic development along with apoptosis changes by confocal microscope. The A and B grade of COCs were recovered from slaughterhouse derived ovaries on the basis of cumulus mass. A total 1229 COCs were recovered from 428 ovaries, out of which 25.14% were of grade A and 44.18% of grade B. In vitro fertilization of grade A COCs produced significantly higher rate of cleavage and blastocyst (89.99±1.49% and 41.99±1.82%) as compared to grade B (81.33±0.81% and 30.66±1.63%). The low rate of embryonic development in grade B COCs might be due to higher incidence of apoptosis (36.66±4.31vs26.66±2.63) as compared to grade A as observed by Annexin-V/Propidium iodide stating under confocal microscope. Thus, our study concluded that the cumulus mass had significant impact on different developmental processes of oocytes, fertilization and embryonic developments.
Key words: Buffalo, cumulus-oocyte complexs (COCs), in vitro fertilization, apoptosis