Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Research Article

JCR. 2020; 7(18): 96-100


RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ANTIDIABETIC DRUGS IN BULK AND PHARMACEUTICAL FORMULATIONS

K.Nagaraju, V. Nagalakshmi, G.Srinivas Rao, K.Showrilu.

Abstract
RP-HPLC method for Simultaneous estimation of bulk and tablet dosage forms. Separation of Alogliptin and
Metformin hydrochloride was achieved ODS C18 (4.6250mm,5m,Hypersil) or equivalent in an isocratic
mode using Acetonitrile :water : Methanol (70:20:10) v/v at a flow rate of 0.8ml/min and elute was observed at
278nm, with a retention time of 1.336 and 2.186 minutes for Metformin and Alogliptin respectively. The
method was linear in the drug concentration range of 1g/ml to5g/ml for Metformin and 1g/ml to 5g/ml for
Alogliptin. The values of the correlation coefficient were 0.986 for Metformin hydrochloride and 0.967 for
Alogliptin respectively. The LOD and LOQ for Metformin hydrochloride and Alogliptin were 0.174 and 0.527
and 0.120 and 0.3099 respectively. The Metformin and Alogliptin recovery percentage is 99 and 100
respectively. The specificity of the method observed the non interference of impurities in sample. As per ICH
guidelines the method is verified with parameters such as Linearity, Accuracy, Precession, Specificity and
Robustness.

Key words: Alogliptin and Metformin hydrochloride, Antidiabetic drugs, RP-HPLCmethod



Similar Articles

New benzyltriethylammonium/urea deep eutectic solvent: Quantum calculation and application to hyrdoxylethylcellulose modification.
Azougagh O, Essayeh S, Achalhi N, El Idrissi A, Amhamdi H, Loutou M, El Ouardi Y, Salhi A, Abou-Salama M, El Barkany S
Carbohydrate polymers. 2022; 276(): 118737

Passive social media use and psychological well-being during the COVID-19 pandemic: The role of social comparison and emotion regulation.
Yue Z, Zhang R, Xiao J
Computers in human behavior. 2022; 127(): 107050

Carbonaceous materials for removal and recovery of phosphate species: Limitations, successes and future improvement.
Recepoglu YK, Goren AY, Orooji Y, Khataee A
Chemosphere. 2022; 287(Pt 2): 132177

A Transfer Learning-Based Approach with Deep CNN for COVID-19- and Pneumonia-Affected Chest X-ray Image Classification.
Chakraborty S, Paul S, Hasan KMA
SN computer science. 2022; 3(1): 17

Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 209-220


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites