Abstract

The study was performed to check the chemical, antioxidant, antimicrobial and protein binding qualities of the hydro-ethanolic extract of Celastrus paniculatus (CP). Hydro-ethanolic extract of CP exhibited low antibacterial and antifungal activities. Excellent antioxidant activities (60-80 %) were noticed for various antioxidant assays viz DPPH (2,2-diphenyl-1-picrylhydrazyl), MC (Metal chelating), FRAP (Ferric reducing), SA (Superoxide radical), and NO (Nitric oxide). Total phenolic content (TPC) and total flavanoids content (TFC) of the extract was 1.38±0.15 mg/g (expressed in equivalents to gallic acid) and 8.91±0.21 mg (expressed in equivalents quercetin) respectively. Moreover, at p ≤ 0.05, significant linear correlation was noticed between the TFC/TPC and antioxidant activities favoured which highlights the medicinal property of CP. Protein binding assay was performed and an observed binding constant (1.11±0.92×10-5 µM-1) was almost close to control (aspirin). Anti-inflammatory assay revealed potent anti-inflammatory activity of hydro-alcoholic extract of CP. These significant findings of CP may provide compelling scientific evidence of its therapeutic potential.

Key words: Antibacterial; Antifungal; Antioxidant; Celastrus paniculatus, Medicinal plants.