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Polymorphism Of Exon 2-3 Of Growth Hormone Gene In Surti and Mehsani Goats by PCR-RFLP

Jyotishree Bayan, Vishnu Kharadi, Umed Ramani, Mamta Janmeda, Pawar Vallabh, Balkrishna Brahmkshtri.


Abstract

The investigation was planned to study GH gene exon 2-3 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon 2-3 region was found to be polymorphic on restriction digestion with Hae III which revealed two genotypes AA and AB with genotypic frequencies of 0.24 and 0.76 in Surti goats and 0.20 and 0.80 in Mehsani goats respectively. Both the population of Surti and Mehsani goats were not found to be in genetic equilibrium for GH locus exon 2-3 indicating selection pressure for growth. Polymorphism in exon 2-3 region of GH gene in both the goat breeds can be utilized in formulating breeding strategies based on marker assisted selection in both the goat breeds.

Key words: Goat, Growth Hormone Gene, Polymorphism, PCR-RFLP.






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