Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

AJVS. 2018; 59(1): 79-91


Characterization of chicken IgY produced against H5 and H9 avian influenza viruses

Mai F. Nahla, Nada A. Abd-elfattah, Madiha S. Ibrahim.

Abstract
Avian influenza viruses are causing sever drawbacks on the poultry industry in Egypt requiring additional approaches together with vaccination to overcome its endemicity. On this study, twelve white Hy-Line layers were immunized with H5N1 and H9N2 viruses. Throughout the experiment (8 weeks), regular blood samples were collected weekly with daily egg collection. The purified IgY was tested for titres and specificity in hens' sera and egg yolk samples using Hemagglutination-inhibition(HI) and neutralization tests(NT). HI test showed that the antibodies titre values against H5N1 and H9N2 increased from the 1st week in the serum and 2nd week in the yolk until reaching a peak at the 4th week in serum and 5th week in the yolk. The peak was maintained over 3 weeks in the yolk of H5N1 and H9N2 and in the serum of H5N1 and over 4 weeks in the serum of H9N2 followed by non-significant decrease until the 8th week. The percentage of transfer of H5N1 antibodies ranged from 6.25% to 50% and for H9N2 ranged from 3.12% to 12.5%. NT showed that the neutralization index of H5N1 and H9N2 were toward homologous 3.9 and 5.04, respectively. Cross NT showed no egg protection with 100% death, but with retardation of the death time. In Conclusion, our results revealed the presence of significant titres of purified IgY against H5N1 and H9N2 influenza viruses in eggs and the possible role of egg yolk IgY in neutralization of avian influenza viruses.

Key words: Avian influenza virus, H5N1, H9N2, IgY, Hemagglutination-Inhibition test



Similar Articles

Growth differentiation factor 5: a neurotrophic factor with neuroprotective potential in Parkinson's disease.
Goulding SR, Anantha J, Collins LM, Sullivan AM, O'Keeffe GW
Neural regeneration research. 2022; 17(1): 38-44

Dendritic spine density changes and homeostatic synaptic scaling: a meta-analysis of animal studies.
Moulin TC, Rayêe D, Schiöth HB
Neural regeneration research. 2022; 17(1): 20-24

Cholesterol synthesis inhibition or depletion in axon regeneration.
Tang BL
Neural regeneration research. 2022; 17(2): 271-276

Clinical Evaluation of the Rapid STANDARD Q COVID-19 Ag Test for the Screening of Severe Acute Respiratory Syndrome Coronavirus 2.
Kim HW, Park M, Lee JH
Annals of laboratory medicine. 2022; 42(1): 100-104

Inhibition of Rhopalosiphum maidis (Corn Leaf Aphid) Growth on Maize by Virus-Induced Gene Silencing with Sugarcane Mosaic Virus.
Chung SH, Jander G
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 139-153


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites