Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

AJVS. 2019; 61(1): 108-118


Evaluation of Antiviral Activity of Allium Cepa and Allium Sativum Extracts Against Newcastle Disease Virus

Rasha Harazem, Sahar Abd El Rahman, Ali El-Kenawy.

Abstract
Newcastle Disease Virus (NDV) is the causative agent of the most devastating diseases affecting poultry especially in large scale production causing severe economic losses due to high mortality which may reach 100% in very virulent strains of NDV. In the present study, field samples of suspected to be infected by NDV were isolated in Embryonated chicken Eggs (ECEs) of 10 days-old via allantoic cavity for three passages, the isolated virus was identified to be NDV by Haemagglutination inhibition test (HI), electron microscope and reverse transcriptase polymerase chain reaction (RT- PCR). The antiviral effects of both onion and garlic extracts were studied on field isolates and reference strain of NDV. The antiviral effects involve the gross lesions changes of the embryo in inoculated ECEs, Haemagglutination test (HA) and Infectivity test (EID50%). The obtained results showed that most of the isolated viruses expressed subcutaneous Haemorrhage and death of the embryo at the fifth day post inoculation via allantoic cavity at the third passage of ECEs, while the field sample number (10) and the reference strain showed extensive haemorrhage and rapid death of embryos. The isolated viruses were identified to be NDV of the susceptible to be infected samples by HI using hyperimmune serum against NDV prepared in rabbit. The Mean Death Time (MDT) was calculated for the field sample number (10) and the reference strain which showed that the tested samples are velogenic strains. Electron microscopy investigation of some selected samples (of high HA titers) identified the virus as pleomorphic in shape. Some selected samples were analyzed by RT-PCR and gel electrophoresis expressing clear bands using primers against the F-gene of NDV. RT-PCR is considered as the most useful, reliable and accuret laboratory test for confirming field NDV. Brown leaves of onion (Allium Cepa) and bulbs of garlic (Allium sativum) were separately extracted and the extracts were tested for their antiviral activity against the previously isolated and identified NDV. Investigating the antiviral effects of both onion and garlic extracts on some field samples showed absence of virus activity after treatments by extracts, while samples number (10) and reference strain showed reduction of virus in HA test, subcutaneous hemorrhage and EID50%. The reduction of NDV infectivity may be due to the blocking of the attachment of the virus with the cell receptors.

Key words: Newcastle disease, Embryonated chicken eggs, HI, Electron microscope, RT-PCR, antiviral effect, garlic extract, onion extract, EID



Similar Articles

Exploring the Alzheimer's disease neuroepigenome: recent advances and future trends.
Zhang H, Elefant F
Neural regeneration research. 2022; 17(2): 325-327

Growth differentiation factor 5: a neurotrophic factor with neuroprotective potential in Parkinson's disease.
Goulding SR, Anantha J, Collins LM, Sullivan AM, O'Keeffe GW
Neural regeneration research. 2022; 17(1): 38-44

Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling.
Bertucci T, Kakarla S, Kim D, Dai G
Methods in molecular biology (Clifton, N.J.). 2022; 2375(): 1-12

Presenilin mutations and their impact on neuronal differentiation in Alzheimer's disease.
Hernandez-Sapiens MA, Reza-Zaldívar EE, Márquez-Aguirre AL, Gómez-Pinedo U, Matias-Guiu J, Cevallos RR, Mateos-Díaz JC, Sánchez-González VJ, Canales-Aguirre AA
Neural regeneration research. 2022; 17(1): 31-37

Highly sensitive detection of multiple proteins from single cells by MoS-FET biosensors.
Wei J, Zhao Z, Lan K, Wang Z, Qin G, Chen R
Talanta. 2022; 236(): 122839


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites