Aporosa wallichii Hook.f. is a medicinal plant, which belongs to Phyllanthaceae family. This plant was used in this study for the determination of several pharmacological activities. This study involved experiments including determination of antioxidant property by performing 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and total phenolic content (TPC) test, determination of cytotoxic activity by brine shrimp lethality assay and thrombolytic property analysis. In this study, it was observed that for DPPH free radical scavenging assay; standard (ascorbic acid) provided IC50 value of 75.6 μg/mL, whereas the extract provided IC50 value of 58.7 μg/mL. In case of TPC test, plant extract showed 308.97 mg of gallic acid equivalents / g of extract. Brine shrimp lethality bioassay revealed cytotoxic property of the plant and the LC50 value of the plant extract was 26.7 μg/mL. Then again, the standard vincristine sulphate provided LC50 value 2.0 μg/mL. Moreover, the thrombolytic analysis showed for standard (clopidogrel) 59.3 % clot lysis and for extract 24.5 % clot lysis. This study revealed that this plant has significant antioxidant activity, moderate level of cytotoxic activity and thrombolytic activity.
Key words: DPPH free radical scavenging assay, Total phenolic content, Brine shrimp lethality assay, Thrombolytic property, Cytotoxicity.
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