Bacterial resistance has a major role in the failure of infectious illness treatments. E.coli is one of the most common causes of mastitis, so a total of 100 samples (50 each from milk and feces) were collected from dairy cows from various farms in Gharbia Governorate Egypt, to determine the prevalence of E.coli, isolates were analyzed for antibiotic susceptibility. The purpose of our research was to assess the antibacterial activity and mechanism of action of two nanoemulsion EOs (Citronella and Mentha spicata) against of Escherichia coli. Antibacterial action was assessed by utilizing the agar well diffusion technique, with an electron microscope used to confirm the effect of minimum inhibitory concentration (MIC) on tested bacterial cell and minimum bactericidal concentration (MBC). E. coli was more susceptible to nanoemulsion EOs with a larger inhibition zone diameter; the present research demonstrates the antimicrobial activity that nanoemulsion EOs have against the tested bacteria by acting on the cell surface and disrupting the cell membrane; bacterial cells treated with both essential oils nanoemulsions demonstrated varying degrees of deformation and disruption. On the surfaces of strains treated with 2 MIC, which was substantially more effective than MIC, considerable morphological alterations were observed.
Key words: Key words: E.Coli, Citronella, Mentha spicata, Essential oils and Electron microscope.
|