Stimulation of immune function is primary focus in the field of cancer metastasis treatment. Many human tumor cells appear to take advantage of the lymphatic system to promote metastasis to lymph nodes and distant organs. In order to treat metastatic cancer, the immune function should be reactivated to recognize the cancer cells against natural killer activity and expose the cancer cells to the chemotherapy. Proteolytic enzymes have been explored in cancer therapy including the inhibition of metastasis and improve the body immune mechanism. To achieve this hypothesis, we proposed papain loaded solid nanoparticles to stimulate the immune mechanism by investigating the following; (i) measurement of human lymphoblast cells using TK6 Spleen lymphoblast cell line (ii) proliferation of T cells and secretion of cytokines (IL-2 and IL-4) using T-lymphocytes 8E5 cells (iii) determination of in-vitro cytokines production by activated macrophages. This research evidenced that papain loaded solid lipid nanoparticles possess significant immunostimulatory efficacy, thus it can be proposed as an adjuvant therapy for cancer metastasis.
Key words: papain enzyme, immune function, lymphoblast cell, cytokines, cancer metastasis, lymphatic system
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