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Original Article



In vitro propagation of chia (Salvia hispanica L.) and assessment of genetic fidelity using random amplified polymorphic DNA and intersimple sequence repeat molecular markers

Anita Yadav, S L Kothari, Sumita Kachhwaha, Anuja Joshi.




Abstract

An efficient micropropagation protocol has been developed for Salvia hispanica L., which bears high nutritional and medicinal value. Seeds of S. hispanica were germinated aseptically on half strength MS medium. Nodal explants obtained from in vitro germinated seedling were cultured on MS medium supplemented with 6-benzyladenine (BAP) (1-5 mg l-1) or Kinetin (Kn) (1-5 mg l-1) individually or in combination with α-naphthaleneacetic acid (NAA) (0.1-1 mg l-1) and indole-3-acetic acid (IAA) (0.1-1 mg l-1) for clonal propagation. It was observed that maximum amount of shoots per explant (9.02±2.65) was obtained on medium supplemented with 3 mg l-1BAP which was also optimum for subculturing of the regenerated shoots. Rooting was obtained on medium containing 1 mg l-1 IBA. The rooted plantlets were acclimatized and transferred to field conditions, with 75% survival rate. Genetic fidelity studies were carried out on regenerated plantlets by RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) as molecular markers.

Key words: In vitro propagation, Chia, genetic fidelity, RAPD, ISSR, Molecular markers






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