Whartonís jelly (WJ) is a gelatinous tissue exists in the umbilical cord contains stromal cells, termed as mesenchymal stromal cells (MSCs). Current study designed to characterize the stem cell population both within the cord blood and itís whartonís jelly tissue and itís correlation to neonatal parameters. Both whartonís jelly and cord blood and their CD34+ and CD90+ expression was enumerated by flow-cytometry. Neonatal parameters along with the mode of delivery were analyzed to correlate with CD34 and CD90 markers. From a total of 20 deliveries, CD34 and CD90 expression pattern showed varied in both samples. Gender and the expression of CD34 and CD90 markers doesnít show any significant change. Whereas, correlation analysis of weight with CD34+ and CD90+ cell counts showed significant difference and it may have the effect on both cell counts. Neonatal parameters especially birth weight may have the effect on cell count of CD34 and CD90 in both CB and WJ. Current study provide a fast method for mesenchymal cell isolation from Whartonís jelly tissue using the total length of the cord, which may be easily applied along with the UC blood, for the banking of UC.
Whartonís jelly; phenotypic characteristics; therapeutic applications; major histocompatibility complex; graft-vs-host disease; umbilical cord.