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Impact of light and dark (L/D) period on the biosynthesis of astaxanthin in green alga Haematococcus pluvialis

Surendra Singh, Ashaq Hussain Rather.

Abstract
The aim of the study was to investigate the impact of light and dark (L/D) period on the biosynthesis of astaxanthin in green alga Haematococcus pluvialis. The astaxanthin was extracted from H. pluvialis with DMSO and the astaxanthin content was determined by the UV-Spectrophotometer. H. pluvialis was incubated under 10:14 h (L/D) period exposed to sunlight at 322oC temperature, the astaxanthin biosynthesis was compared with the culture which was incubated in controlled air-conditioned culture room of 16:8 h (L/D) period at 252oC. Highest astaxanthin content was found as 0.045 g mL- 1 in H. pluvialis culture which was exposed to sunlight under 10:14 h (L/D) on 9th d of study. Under 16:8 h (L/D) period at 25 2oC, the highest astaxanthin content was found as 0.04 g mL-1 on 17th d of study. From our present investigation, it is apparent that the 10:14 h (L/D) period is more effective in promoting astaxanthin content in green alga Haematococcus pluvialis.

Key words: Haematococcus pluvialis, Astaxanthin, Biosynthesis



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