Home|Journals|Articles by Year|Audio Abstracts

Original Article

Artocarpin isolated from Artocarpus heterophyllus heartwoods alters membrane permeability of Streptococcus mutans

Abdi Wira Septama, Pharkphoom Panichayupakaranant.


Artocarpin was successfully isolated from the heartwoods of A. heterophyllus. Objectives: The present study was conducted to determine antimicrobial activity of artocarpin against cariogenic microbial including Streptococcus mutans, Escherichia coli, and Candida albicans. The activity of artocarpin on alteration of cell membrane was also investigated. Methods: Standard microdilution method was applied to evaluate its antibacterial activity against tested microorganisms. Time-killing test was then performed to verify its antimicrobial effect. In order to know its effect on membrane permeability, bacteriolysis assay was selected, followed by determining loss of 260 nm absorbing material using UV-Vis spectrophotometer. Results: Artocarpin exhibited strong antibacterial against S. mutans with MIC and MBC values of 1.95 and 3.91 µg/mL, respectively. Time-killing curve showed that artocarpin at 2 × MIC (3.9 μg/mL) and 4 × MIC (7.8 μg/mL) inhibited bacterial growth after 4 h incubation which was comparable to ampicillin as a positive control. Artocarpin 2 × MIC (3.9 μg/mL) and 4 × MIC (7.8 μg/mL) altered membrane permeability of S. mutans after 24 h exposure and led to release 260 nm absorbing material as an indicator of membrane cell damage. Conclusion: This finding indicated that artocarpin has high potency as antibacterial compound against S. mutans by reducing cell membrane permeability.

Key words: Artocarpin, A. heterophyllus, antimicrobial, membrane permeability, S. mutans

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.