Home|Journals|Articles by Year|Audio Abstracts

Original Research

RMJ. 2018; 43(4): 772-775

Hepatocytes of liver lobule are effected by Sodium arsenite and Lagenaria siceraria has protective role

Saima Qureshi, Khadija Qamar, Afnan Gul.


Objective: To assess the effects of Lagenaria siceraria on arsenic induced fatty change in hepatocytes of liver.
Methodology: The study was done at Anatomy Department, Army Medical College Rawalpindi in collaboration with National Institute of Health (NIH) Islamabad for a period of eight weeks. Sprague Dawley rats (both male and female separately housed) were divided randomly into five groups each group indiscriminately had 10 animals. A and B served as untreated controls and C, D and E were experimental groups. Experimental groups were intervened with sodium arsenite to induce hepatotoxicity. Experimental group C was sacrificed after four weeks of arsenic intervention to observe immediate effects. In the next four weeks Group D was left without intervention to observe whether the toxic effects were self-limiting. Concomitant use of Lagenaria siceraria on group E along with arsenic was done. Liver specimens were stained with H & E and semi qualitative scoring of steatosis was done according to Brunt steatosis scoring. Data analysis was done by SPSS v 22.
Results: In group C animals, the fatty change (steatosis) was extensive and marked as compared to the control group A. Steatosis was also observed in the experimental groups D and E but it was less marked. In group E, the fatty change decreased extensively after the concomitant use of lagenaria siceraria (p≤0.05).
Conclusion: Administration of arsenic induced fatty changes in the hepatocytes. Concomitant use of Lagenaria siceraria mitigated these fatty changes in hepatocytes.

Key words: Sodium arsenite, steatosis, Lagenaria siceraria

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Review(er)s Central
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.