Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Research

Prevalence and impact of Carbapenemase-encoding genes in Clinical isolates of Carbepenem-Resistant Pseudomonas aeruginosa

Ayman Kamal El Essawy, Hala Mohamed Abu Shady, 16- Bassem Aly Mahmoud El- Biaa.

Multidrug resistant Pseudomonas aeruginosa (MDRPA) and carbapenem-resistant Pseudomonas auruginosa (CRPA) are difficult to treat. Carbapenem resistance is due to multiple mechanisms and among these mechanisms production of carbapenemases is the most important. The aim of this study was investigating the prevalence of carbapenemase encoding genes that were associated with CRPA. Bacterial isolation, identification and antibiotic sensitivity were performed. Selected CRPA isolates were investigated for the presence of specific carbepenemase- encoding genes. Gene mutation was enhanced by exposure of the bacterial cells to UV radiation. Among the investigated CRPA isolates (12/22, 54.5%) were harboring genes for carbapenemases production. KPC, VIM, IMP genes were detected in (11/22, 50%), (5/22, 22.7%) and (4/22, 18.2%) respectively, while SME, NDM-1(using two pairs of primers) and OXA genes were not detected in any of the tested CRPA isolates. In the absence of KPC, IMP and VIM genes in bacterial mutants, MIC for carbapenems were decreased from ≥16 to ≤2 mg/L. The prevalence of cabapenamase encoding genes was relatively high in the current study, which was reported in association with high mortality rate. In contrast to a recent publication, KPC and IMP genes were detected in Kuwait hospitals and their impact on resistance was proved.

Key words: Carbapenem resistance; Pseudomonas aeruginosa; carbapenemase encoding genes

Similar Articles

Functionality of a bicistronic construction containing and genes encoding β-hexosaminidase A for cell-mediated therapy of GM2 gangliosidoses.
Shaimardanova AA, Chulpanova DS, Solovyeva VV, Aimaletdinov AM, Rizvanov AA
Neural regeneration research. 2022; 17(1): 122-129

Investigating the role of imprinted genes in pediatric sporadic brain arteriovenous malformations.
Scimone C, Donato L, Sidoti A
Neural regeneration research. 2022; 17(1): 101-102

Isolated heterotrophic nitrifying and aerobic denitrifying bacterium for treating actual refinery wastewater with low C/N ratio.
Hu J, Yan J, Wu L, Bao Y, Yu D, Li J
Journal of bioscience and bioengineering. 2021; ():

Development of 'multiresistance rice' by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system.
Li C, Zhang J, Ren Z, Xie R, Yin C, Ma W, Zhou F, Chen H, Lin Y
Pest management science. 2021; 77(3): 1536-1547

Clinical Contribution of Next-Generation Sequencing Multigene Panel Testing for BRCA Negative High-Risk Patients With Breast Cancer.
Ece Solmaz A, Yeniay L, Gökmen E, Zekioğlu O, Haydaroğlu A, Bilgen I, Özkınay F, Onay H
Clinical breast cancer. 2021; ():

Full-text options

Add your Article(s) to Indexes
• CiteIndex: Articles & Statistics

American Journal of Diagnostic Imaging


Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.