Association between mangrove plant with fungi has been explored for many biological activities. The objective of this study is to screen the antimicrobial and cytotoxic activities of endophytic fungi isolated from leaves, bark, and roots of mangrove Scyphiphora hydrophyllacea C. F. Gaertn collected from Teluk Pandan, Bungus, West Sumatra, Indonesia. The isolation of endophytic fungi was conducted by direct planting on growing medium Sabouraud Dextrose Agar (SDA) and obtained eleven isolates of the fungus. The isolates were cultivated on rice medium for one month and extracted using ethyl acetate. The 11 ethyl acetate extracts were screened for cytotoxic activity by Brine Shrimp Lethality Test (BSLT) method and MTT assay and for antimicrobial activity toward some human pathogenic microbial. The results of this study indicate that five (45.5%) isolates of endophytic fungi can inhibit the growth of Staphylococcus aureus, Escherichia coli, dan Candida albicans with a value ranging from 9.1-21 mm. While 4 extracts (36.4%) were cytotoxic (cell viability < 50%) against T47D cells. The potential fungal isolate ADSH2 was identified as Penicillium chrysogenum by molecular characterization. The results of this study indicate that endophytic fungi associated of Scyphiphora hydrophyllacea can be developed as of antimicrobial and anticancer compounds.
Key words: Antimicrobial activity, Cytotoxic activity, mangrove, Penicillium chrysogenum, Scyphiphora hydrophyllacea