Abstract

Background & Aims:
Healthcare-associated infections remain a major threat in intensive care units, where environmental reservoirs promote pathogen persistence and antimicrobial resistance. Despite national infection-control programs, limited data exist on surface contamination in intensive care units in Jazan, Saudi Arabia.
Methods:
A cross-sectional study was conducted in intensive care units and pediatric intensive care units of a central hospital and a general hospital. Sixty-five swab samples were collected from five high-touch surfaces per bed—bed rails, ventilator panels, cardiac monitors, and infusion pumps. Samples were cultured on blood and MacConkey agars; isolates were identified using standard microbiological methods and the VITEK system. Antimicrobial susceptibility was assessed by the Kirby–Bauer method following Clinical and Laboratory Standards Institute guidelines.
Results:
Overall, 76.9% of samples yielded bacterial growth on blood agar and 21.5% on MacConkey agar. Bed rails were the most contaminated (>90%), followed by ventilator panels and monitor keypads. Seventy-eight isolates were recovered, dominated by Enterobacteriaceae (62.8%) and Pseudomonas spp. (29.5%)Staphylococcus aureus (including two methicillin-resistant isolates) and Candida species accounted for 15.4% and 3.8%, respectively. CH showed higher overall contamination (88%) than GH (52%, p < 0.05), while GH exhibited a higher proportion of Gram-negative isolates (32% vs. 16%). Among Enterobacteriaceae, 70% were resistant to ampicillin, 40% to gentamicin, and 25% were multidrug-resistant. Pseudomonas showed 30% piperacillin resistance, and all methicillin-resistant Staphylococcus aureus isolates were oxacillin-resistant.
Conclusion:
Intensive care unit environments in Jazan hospitals display contamination with clinically relevant and resistant bacteria. Strengthened cleaning protocols, targeted disinfection of high-touch surfaces, and enhanced antimicrobial stewardship are essential to mitigate Healthcare-associated infections and antimicrobial resistance transmission.

Key words: Healthcare-associated infections, Intensive care unit, Environmental contamination, Antimicrobial resistance