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Majmaah J Heal Sci. 2014; 2(1): 41-46

Relativity of Platelet Count with Automated Cell Counter & Manual Haemocytometer using Peripheral Blood Smear

Purnendu Sinha, Moattar Raza Rizvi, Ranjay Kumar Choudhary.


Background: Obtaining accurate, precise, and reliable platelet counts as a reference for the calibration of hematology analyzers has been a continuing problem due to varying results. We proposed the relativity of platelet count by Automated Cell Counter and Manual Haemocytometer with Peripheral smear for focusing the accuracy and & cost effectiveness, without compromising the accuracy of test results.

Methods: Seventy-Five blood samples in EDTA-anticoagulant vacutainer tubes were obtained and randomly divided into 3 group; Group I-normal control (n = 25), Group II-mild thrombocytopenia (n = 25) and Group III-severe thrombocytopenia (n = 25). Each blood sample had two peripheral blood smears made and stained on an automatic slide stainer. Peripheral blood smears were analyzed for platelet count by manual microscopy, an automated hematology analyzer Beckman Counter Diff 2 in all groups. The agreement between the two methodologies was assessed using the paired t-test and correlation coefficient analyses.

Results: The coefficient of variation values significantly differed in Group C and was high for automated cell counter. High positive correlation was also observed between Automated cell counter values and manual method (r=0.82, p

Key words: Thrombocytopenia peripheral blood smear, platelet count, automated Cell Counter and haemocytometer

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