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Nig. Vet. J.. 2018; 39(3): 289-291

Evaluation of Salmonella Isolates Obtained from Poultry farms in Abia and Imo States for presence of plasmid.

Nwiyi Okechukwu Paul, Chah Founkition Kennedy, Shoyinka Sola Victor.


The study was conducted to determine whether there exist the presence of plasmid from Salmonella isolated from poultry farms in Abia and Imo states southeast Nigeria. Forty Salmonella isolates was used for the study. The alkaline phosphate method of Birnboin and Doly was employed. Three buffers A, B and C made up of different concentration and volume with adjusted pH were used. A (400mM Tris, 200mM Na EDTA, acetic acid), B (3M Na, acatic acid and C (10mM Tris, 2M Na EDTA, acetic acid). The test organisms sub-cultured in MacConkey agar (MCA) was processed at various centrifugation rates and time. The suspected pure plasmid DNA was mixed with ethidium bromide and loading dye using agarose. The set up was viewed under UV transillumination with gel documentation apparatus. Plasmid cure was conducted with ten multidrug resistant Salmonella isolates. Danifor Biotechnology method was used for the plasmid curing experiment. Three curing agents, SDS curing agents (100), SDS (10g or 10%) and nutrient broth (100ml) were used. Plasmid DNA was detected from the Salmonella isolates evidenced with bands at 100bp. There were plasmid cure as supported by the zones of inhibition by some of the antibiotics when compared with the original isolates.

Key words: Keywords: Cure, DNA, multidrug resistant isolates, plasmid, Salmonella.

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