The objective of our study was to evaluate the secondary metabolites produced from callus culture of Pelargonium sidoides. This study was performed to evaluate,1) the growth of Pelargonium sidoides DC. calli derived from different explant tissues (leaf, root and shoot tip), 2) to determine total phenolic, flavonoid and proanthocyanidin contents in aqueous methanol, water and aqueous acetone extracts of calli from different explants. Moreover, the antimicrobial activities of above different extracts against food borne diseases caused by pathogenic bacteria were studied. The medium which contained 1.0 mg/l α-naphthalene acetic acid and 0.2 mg/l Kinetin was best to get friable calli with little brown deposits. The highest phenolic content was in calli from root extracted with aqueous acetone followed by calli from shoot tip extracted with the same solvent (12.34 and 4.78 mg/g DW) respectively. Also, the highest values of flavonoid content (20.82 mg/g DW) and Proanthocyandins (0.290 A550 nm/mg) were recorded with calli from root extracted with aqueous acetone. Antimicrobial effect of different calli extracts showed that, the maximum inhibition zone diameter (35mm) was recorded with callus from shoot tip explants extracted with aqueous acetone against Clostridium perfringens ATCC.
Key words: Pelargonium Sidoides, Callus growth, Phytochemical analysis, Antimicrobial activities.
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