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Evaluation of Myc Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic in-Situ Hybridization (Dual CISH) Assay

Daniel Lerda, Marta Cabrera, Jorge Flores, Luis Gutierrez, Armando Chierichetti, Martin Revol, Hernan Garcia Önto.


Objetive: The overall purpose of the study was to demonstrate applicability of the Dako
dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup)
with respect to fluorescence in situ hybridization (FISH) probes MYC-C.
Methods: MYC gene amplification by FISH and Dako dual-color CISH
Results: The study showed that the dual-color CISH assay can convert Texas red and
fluorescein isothiocyanate (FITC) signals into chromogenic signals. The dual –color
CISH assay was performed on 40 cases of prostate cancer. Amplification was identified
in 12 of 40 (30%) tumors. No amplification was seen in 28 of 40 (70%) tumors. FISH
data were available in total of 40 tumors. All tumors showed concordant results between
dual-color CISH and FISH for classifying a tumor as MYC amplified or not amplified.
Conclusions: We conclude that dual-color Dako CISH assay is an accurate method for
determining MYC gene amplification with added advantages that make it a more
practically useful method.

Key words: MYC, prostate cancer, chromogenic in situ hybridization, CISH

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