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Effect of Ascorbic Acid and Alpha-Tocopherol on Expression and Quantification of Heat Shock Protein 70 (HSP70) by Western Blotting During Heat Stress in Commercial Broilers

Amir Amin Sheikh, Aditya Mishra, Rakshanda Bhagat, Kailash Kumar, Jaan Mohammad Wani, Gowher Gull Sheikh.

Abstract
The present study was carried to investigate the effect of ascorbic acid and alpha-tocopherol on expression and quantification of heat shock protein 70 (HSP70) during heat stress in commercial broilers by western blotting. A total number of 168 day old Cobb 400 commercial broiler birds were randomly divided into seven groups and each group consist of 12 birds in two replicates. Work was done in two conditions heat and comfort. Heat stressed groups were maintained at 375.0C where as Comfort groups were maintained at 261.0C. G1 (comfort) and G8 (Heat) group was kept as control. 100 mg, 200 mg and 300 mg ascorbic acid was supplemented along with feed in G2, G3 and G4 and 100 mg, 200 mg and 300 mg alpha-tocopherol was supplemented to G5, G6, G7 groups respectively. On western blot expression analysis it was found that minimum HSP70 expression was observed in 200 mg ascorbic acid and 300 mg alpha-tocopherol supplemented groups

Key words: Alpha-tocopherol, Ascorbic acid, Broilers, Heat stress, HSP70, Western blotting



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