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Fixation of T Allele in C>T Polymorphism in Intron IV Region of Secreted Phosphoprotein 1 (SPP1) Gene in Indian Cattle Breeds

Mona Sharma, Satyendra Pal Singh, Madhu Tiwari, Deepak Sharma, Avneesh Kumar, Rakesh Goel, Brijesh Yadav.

Abstract
Secreted phosphoprotein 1 (SPP1) is a highly negative phosphorylated glycoprotein, which has potent role in growth, production and reproduction of the animals. In the present study, investigation of SPP1 gene polymorphism was undertaken in 147 Sahiwal and Hariana cattle maintained at Instructional livestock farm complex (ILFC), DUVASU, Mathura using BsrI/PCR-RFLP assay. Amplification of SPP1 intron IV (C>T) region revealed 290 bp product and restriction digestion with BsrI showed only one type of genotypes, namely, TT (290 bp). The frequency of TT genotypes was 100% in all screened samples with T allele (1.0). Association studies of SPP1 gene with milk production traits could not performed because of monomorphic pattern of genotypes.

Key words: Sahiwal, Hariana, BsrI, PCR-RFLP, SPP1, Intron IV, Milk Production Traits



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