Abstract

Sub-fertility is one of the most common problems in men. Neutrophils represent the first immune cells arriving at the site of inflammation, and they turn into pus cells at the site of inflammation. Thus, the present study aimed to study the association of pus cells with sperm cell abnormalities and the subsequent effect on men’s fertility in patients with sub-fertility. The current study included 50 samples of healthy individuals and 125 samples collected from sub-fertility patients. Semen parameters were measured. Moreover, the enzyme-linked fluorescent assay (ELFA) was used to measure testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and thyroid-stimulating hormone (TSH) levels. The association of pus cells with sperm cell abnormalities and aggregation was also studied. The results found that the semen volume and viscosity were greatly decreased (p≤ 0.05) in the patient group. The sperm cell aggregation increased by 80% in patients with sub-fertility. A clear-cut increase in the levels of pus cells was observed in the patient group. The levels of sperm cells were significantly decreased in the progressive motile (PR) grade in the patient group. Moreover, the abnormalities of the sperm cells were greatly increased in the sperm head, neck, and tail in the patient group. The present results found no significant differences in the levels of T, LH, FSH, PRL, and TSH. Furthermore, the results found a strong association between pus cells and sperm cells abnormalities (R² = 0.7304, p

Key words: Pus cells, sub-fertility, semen parameters, fertility hormones.