Bacterial resistance to antibiotics of Beta lactam group is increasing worldwide threatening the public healthcare due to the limited treatment substitutions. Beta lactamase especially Extended Spectrum Beta Lactamase (ESBL) enzymes are responsible for the most important resistance mechanisms of the Enterobacteriacae to antibiotics of Beta lactam group. In this study, in order to investigate the prevalence of extended-spectrum β-lactamase among Enterobacteriacae isolated from clinically mastitis and apparently normal cattle. A total number of 500 milk samples were collected from cow milk. A total number of 100 ESBL Enterobacteriaceae isolates were obtained. Bacterial isolates were identified by VITEK 2 compact and then ESBL enzymes were identified phenotypically. Estimation of sensitivity of these isolates against variety of antibiotics by Disk Diffusion Sensitivity Test was carried out. Finally ESBL enzymes were identified by Real Time Polymerase Chain Reaction (rPCR). The results revealed that, out of the 100 Enterobacteriacae, 17 isolates (17 %) were positive phenotypically for ESBL. Of which, 14 isolates were positive for genotypically for ESBL. Furthermore, it was found that CTX-M Gene is the most prevalent gene in the Enterobacteriacae isolates
Key words: Enterobacteriacae,antibiotic resistance ,
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