Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Research

IJHRS. 2012; 1(2): 87-93

Localized Chromosomal Radiosensitivity as a Biomarker in Amenorrhea with Normal Karyotypes

Chitra Sankari Kuppuswamy, Hindumathy Chelvaraya Kannan.

Background: The frequency of chromosomal breaks and deletions is higher in cases of primary amenorrhea of gonadal dysgenesis (46, XX) followed by Turners. Karyotype studies may be performed in blood or gonadal tissue or peripheral blood.

Objective: To analyze the radiosensitivity of the chromosomes from patients with primary amenorrhea and compare them to their respective control.

Materials and Methods: The patients studied were classified into 3 groups consisting of 12 patients with 45, X complement (Turner’s syndrome), patients with 46, XX karyotype (gonadal dysgenesis) and a control group of 10 age matched healthy female individuals with proven fertility and with normal karyotype.

Results: The relative radio sensitivity of the individual chromosomes was expressed as the ratio between the patients and the control. When the ratios for any specific chromosome is 1.5 or greater are considered as sensitive chromosomes for radiation. Among primary amenorrhea, radiosensitivity of the chromosomes is significantly higher in patients of gonadal dysgenesis when compared (0.95 ± 0.08) with the control (0.58 ± 0.0646). Chromosome 7 and X showed a significantly high number of breaks at 7q22 and Xq22 respectively. Out of a total number of 167 breaks on No.7 and 178 breaks on X, 67 breaks were on 7q22 and 59 were on Xq22 respectively.

Conclusion: Thus, it may be concluded that both the chromosomal variants of Turner syndrome, namely, 45,X and 46,XX gonadal dysgenesis patients are significantly more radiosensitive than the normal controls. This study shows that radiation can be used as a tool to diagnose genetic disorders in man.

Key words: Primary amenorrhea, Chromosomes, Radiosensitivity

Similar Articles

Primary Isolation of Mycobacterium ulcerans.
Yeboah-Manu D, Asante-Poku A, Yaw Aboagye S
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 17-28

Xie X
Journal of mathematical analysis and applications. 2022; 505(2):

Characterization of Smoothened Phosphorylation and Activation.
Jia J, Han Y, Cho YS, Jiang J
Methods in molecular biology (Clifton, N.J.). 2022; 2374(): 121-137

The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.
Yang S, Zheng X
Methods in molecular biology (Clifton, N.J.). 2022; 2374(): 89-94

Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling.
Kinnebrew M, Johnson KA, Radhakrishnan A, Rohatgi R
Methods in molecular biology (Clifton, N.J.). 2022; 2374(): 73-87

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.

ScopeMed Web Sites