Abstract: Fluoride causes major human health complications and evidence suggests that exposure of fluoride during developmental period adversely affect neurodevelopment, behavior and maturity of brain as this periods are critical for developing stages for CNS. The present study aimed to assess the mechanically induced pain sensitivity, cell morphology, circuitry in terms of neural connections and networks and maturation of neurons under fluoride induced toxicity with concurrent treatment of protective effects of Abelmoschus moschatus seed extract from day 1 of pregnancy to post-natal day (PND) of rat with age 30th day. Timed pregnant wistar rats (30) were segregated into six groups, viz. control, sodium fluoride (NaF) (20 ppm), NaF + A. moschatus aqueous extract (AMAE), NaF + A. moschatus ethanolic extract (AMEE), AMAE, and AMEE and treated for 51 days (21 gestational and 30 post-natal days). On post-natal day 1, 7, 14, 21 and 30, rats were assessed for oxidative stress markers (GSH and GSSG) and neurohistology of brain. Increased threshold levels to mechanical stimulus was observed in fluoride treated rats. Brain stained with Congo red, Cresyl violet and Golgi cox for β-amyloid, Nissl substance and synaptic connections respectively showed cells become amyloidosis with decreased Nissl substance and decreased number of neuronal connections in NaF exposed rats. Reduced content of GSH and increased GSSG levels (P
Key words: Abelmoschus, Cresyl violet, Fluoride, β-amyloid, Nissl substance, Neurodegeneration.
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