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Comparision of Five Different Enrichment Broth-Agar Combinations for the Isolation of Campylobacter jejuni

Savita R Kashappanavar, Archana S Nair, H D Vanitha, Binsy Mathew, Sunil Baskaran, Latha C Sudheesh, Vrinda Menon.




Abstract

Campylobacter is a fastidious microaerophilic bacterium, it is a leading cause of bacterial gastroenteritis in humans globally. In the present study, the efficacy of five enrichment broths in combination with five selective agars for the isolation of Campylobacter jejuni was compared to identify a best possible combination of broth- agar for enrichment. Where, C. jejuni standard culture (ATCC®33560 TM) was enriched in five enrichment broths viz., Brucella, Preston enrichment, modified Charcoal Cefoperazone Deoxycholate broth (mCCDB), Park and Sanders, and Bolton broths, and consequently plated onto modified Charcoal Cefoperazone Deoxycholate agar (mCCDA), Karmali agar, Columbia blood agar, Campylobacter Cefex agar and Campylobacter agar base (CAB). Bolton broth and mCCD agar combination showed the maximum enrichment efficiency and the colony recovery by Bolton broth significantly differed when compared to the Park and Sanders and Brucella broths. The result of the current study shows the combination of enrichment broth and agar most appropriate for the isolation of C. jejuni.

Key words: C. jejuni, mCCDA, Karmali, Bolton, Preston Enrichment






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