Ethyl acetate fungal extracts derived from the marine sponge Acanthostrongylophora ingens, were tested for cytotoxic activity against WiDr, and Vero cell lines. Three of fungi extracts exhibited strong cytotoxicity with the lowest percentage of viability (≤ 50%) occurring at concentrations of 100 µg/mL. One isolate (IB141) showed specific cytotoxicity against WiDr cells, but not against Vero cells. This isolate was identified based on molecular characterization using sequence analysis of the partial 18S rRNA gene. The result indicated that IB141 was identical to Aspergillus ochraceus. A relatively high proportion of positive bioactivity screening results were obtained in this study, demonstrating that the marine derived fungi from the sponge A. ingens have potential as a source of new anticancer agents.
Key words: Acanthostrongylphora ingens, Aspergillus ochraceus, Cytotoxic activity, Marine sponge-derived fungi, Molecular identification