Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Research

Nig. Vet. J.. 2019; 40(2): 166-177


Occurrence of Mastitis and methicillin resistant Staphylococcus aureus in Goats in Zaria, Kaduna State, Nigeria

Ekaette Constant Udoh, Jacob K.P. Kwagaj, Veronica Jarlath Umoh, Moshood Abiola Raji.

Abstract
The objective of this study was to conduct a cross sectional study to assess the occurrence of mastitis and Methicillin Resistant Staphylococcus aureus (MRSA) in goats in Zaria, Kaduna State from May to July 2013. A total of 386 fresh goat milk samples from both right and left halves of 198 lactating goats from National Animal Production and Research Institute (NAPRI) and other locations within Zaria metropolis were examined for the occurrence of mastitis and MRSA. The observed prevalence of mastitis in goats, defined by positive (≥+1) California Mastitis Test (CMT) was 119 (60.1%). The prevalence of mastitis showed significant variation among goats of different breeds (p=0.048) but no significant variation between age groups (p=0.890), location and goats raised under the two different systems of management (p=0.438). Also, there was no significant difference as to which udder half was most sub clinically affected (left or right). From a total of 386 fresh goat milk samples examined, 60 strains of staphylococci were isolated and identified using the conventional biochemical tests and 32 of which were identified as Staphylococcus aureus using the MicrogenTMSTAPH-ID system. Furthermore, 12 of these 32 showed agglutination with the PBP-2a Latex agglutination test reagent. The susceptibilities of the isolates to 14 antimicrobial agents were evaluated using the Kirby-Bauer disc diffusion method and interpreted as sensitive, intermediate or resistant following the Clinical and Laboratory Standards Institute (CLSI, 2011). Out of the 32 isolates of S. aureus ; 90.63% were resistant to cefoxitin, Penicillin G (93.75%), Ampicillin (100%), Amoxicillin-clavulanic acid (34.38%), Ceftriaxone (71.88%), Vancomycin (31.25%), Gentamicin (31.25%), Kanamycin (46.88%), Erythromycin (43.75%), Tetracycline (40.63%), Ciprofloxacin (3.13%), Nitrofurantoin (15.63%), Trimethoprim-sulfamethoxazole (25%) and Chloramphenicol (15.63%). All the isolates were resistant 2 or more antimicrobial agents, but none was simultaneously resistant to all. There were 28 antimicrobial resistant patterns in which resistance to Ampicillin, Penicillin G, Cefoxitin were the most frequent. Methicillin resistance was determined using Cefoxitin discs as a surrogate marker of mecA gene as recommended by Clinical Laboratory Standards International (CLSI). In conclusion, the findings of this study confirm the importance of S. aureus as a mastitis causing organism and the possible role of goats in the transmission of multiple drug resistant S. aureus.

Key words: mastitis, methicillin resistant Staphylococcus aureus, goats, Zaria.



Similar Articles

Synthesis of enhanced corrosion resistant Fe-B-C-Ti amorphous ribbons and evaluation of their photodegradation efficiency under light irradiation.
Zuo M, Moztahida M, Lee DS, Yi S
Chemosphere. 2022; 287(Pt 2): 132175

Multidrug-Resistant Infections in the Developing World.
Singh P, Holmen J
Pediatric clinics of North America. 2022; 69(1): 141-152

Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans.
Thomas SS, Kalia NP, Pethe K
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 209-217

Fluorescence detection of Staphylococcus aureus using vancomycin functionalized magnetic beads combined with rolling circle amplification in fruit juice.
Wang Y, Wang Z, Zhan Z, Liu J, Deng T, Xu H
Analytica chimica acta. 2022; 1189(): 339213

Synergistic inactivation of Escherichia coli O157:H7 and Staphylococcus aureus by gallic acid and thymol and its potential application on fresh-cut tomatoes.
Zhang X, Zhou D, Cao Y, Zhang Y, Xiao X, Liu F, Yu Y
Food microbiology. 2022; 102(): 103925


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites