Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Dose response study of a novel gold-manganese oxide nano-composite in an in vivo murine model

Anupam Nath, Ramkrishna Pal, Leichombam Mohindro Singh, Himadri Saikia, Hasimur Rahaman, Sujit Kumar Ghosh, Ritwik Mazumder, Mahuya Sengupta.

Abstract
In this study the multifunctional property of gold-manganese oxide nano-composites has been utilized to assess their prospect in living organisms. The nano-composite has been synthesized at a water/n-heptane interface under mild reflux conditions for desired functionalization. Nanomaterials have tremendous potential as biological response modifiers largely due to their novel properties in the quantum domain. However, it is imperative to have an improved understanding of the potential risks associated with exposure to nanomaterials as these can detrimentally affect the living system. Therefore, in our present study, we have performed few in vivo toxicity analyses in a murine model to determine their sub-lethal dose, so that subsequent applications can be effective but non-hazardous. Toxicity profiling is performed for the particle using parameters that include bio-accumulation assay by inductively coupled plasma atomic emission spectroscopy (ICP-AES), liver and kidney function assays by blood serum profiling, routine hematological study and histopathological analyses by hematoxylin and eosin (HE) staining. ICP-AES data reveal significant accumulation of metal in liver and kidney for doses 1.3x10-3 mg/kg body weight of mouse and higher. Hepatic function tests indicate atherosclerosis while renal function assays show significant abnormalities at such doses. From the hematological study, a clear indication of steatosis is observed at the above mentioned doses. The red blood corpuscle (RBC) count represents an IC50 value of 4.3x10-4 mg/kg body weight of mouse. From the present study, therefore, a sub-lethal dose lower than IC50 value can be used for future applications.

Key words: gold-manganese nanocomposite; in vivo toxicity; murine model; hematological and serum profiling; histopathology; bioaccumulation.



Share this Article


Advertisement
Journal of Behavioral Health

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.