Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

Punam Yadav, Suresh Korpole, Gandham S Prasad, Girish Sahni, Jyoti Maharjan, Lakshmaiah Sreerama, Tribikram Bhattarai.

Abstract
The present study was conducted to identify and characterize the thermophilic bacteria isolated from five hot springs, viz., Sinkosh, Singha, Bhurung, Ratopani and Paudwar located in Myagdi district, Nepal by using phenotypic and genotypic methods. The hot spring has temperature 420-62 0C and pH 6.5-6.8. Isolation of thermophiles was done by using simple enriched nutrient broth media at 60 0C.Selected strains were screened for thermostable enzymes; cellulase, hemicellulase, amylase, protease, gelatinase, and lipase using substrates carboxymethyl cellulose (CMC), xylan, soluble starch, casein, gelatin and Tween (20,40,60 or 80) respectively. The bacteria were grouped in sixteen groups based on morphological and biochemical characteristics. 16S rRNA sequence analysis of sixteen isolates, one isolate representing one group and phylogenetic analysis showed the cluster of five distinct taxonomic groups. The groups were identified as for genus Anoxybacillus, Aeribacillus, Brevibacillus, Bacillus, and Geobacillus, based on ≥ 95% similarity with reference strains. This is the first study that reports Anoxybacillus sp. Brevibacillus sp. and Aeribaceillus sp. from the hot springs of Nepal.

Key words: Thermostable enzymes, amylase, cellulase, xylanase, hot springs, Anoxybacillus, Biochemical



Full-text options

Full-text Article




Advertisement
American Journal of Preventive Medicine and Public Health

SUBMIT YOUR ARTICLE NOW




ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.