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Determination of acrylamide induced chick embryo brain glutathione S-transferases expression through enzyme activity and western blot

Sreenivasulu Dasari, Sailaja Gonuguntla, Balaji Meriga, Thyagaraju Kedam.

Glutathione S-tranferases (GST) are major detoxification enzymes which belongs to phase II defence enzymes; they can able to metabolize variety of toxic chemical agents like carcinogens, genotoxins, neurotoxins, pesticides etc. Usually, GST will express when the living beings are encountered toxic chemical compounds. Acrylamide (ACR) is synthesized industrially and widely used in various industries. Usually, ACR formation occur food products prepared at high temperature. So that ACR is an environmental and food contaminant and it is well proven neurotoxin. Because of highly mobile nature birds that include poultry birds are main victims to xenobiotics (e.g., ACR) via food, water and agricultural chemical formulas. In this study, ACR administered chick embryo brain GST activity level was assayed using 1-chloro 2,4-dinintrobenzene (CDNB) and expression was assessed by western blot studies. The results show that the GST expression levels were increased in response to ACR by twenty four and forty eight hours intervals. But, in forty eight hours interval GST expression levels decreased slightly. Western blot studies also shows similar pattern of GST expression. Immune blot studies showed similar GST band pattern as purification studies showed (our published work). In this study, enzyme activity and western blot analysis proved that the chick embryo brain GST was expressed more to detoxify ACR.

Key words: Key words: Chick embryo brain GSTs, acrylamide, GST expression, western blot

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