Natural substances extracted from plants have been gaining attraction as protective agents due to their safety and responsible for multiple biological effects on skins. The present work evaluates the photo protective potential of different solvent extracts of Graptophyllum pictum leaves, and the comparison of its SPF value against photo-stability and thermal-stability under a 21-day of storage process. Preliminary phytochemical screening was also performed. The dried powdered leaves were extracted by cold maceration method, using five different solvent, i.e. methanol, ethanol, chloroform, ethyl acetate and hexane. The sun protection factors of all extracts were analyzed by ultraviolet (UV) spectrophometry according to Mansurs method. The SPF results of the five types of extracts were found to have significant differences (P < 0.001). Methanol extract displayed the highest SPF value (15.303±0.045), while the lowest SPF value was showed by ethyl acetate extract. SPF reductions of all extracts under sun exposure condition were higher compared to the extracts placed in dark conditions at room temperature. As compared, the ethanol extract displayed better chemical and thermo-stability with higher in-vitro SPF, at 200 µg/ml. These findings suggested that ethanol was the solvent of choice for yielding high levels of photo-protective ingredients which can be used to formulate effective sunscreen preparations.
Key words: photo protective potential, Sun Protection Factor, Graptophyllum pictum, leaves, solvent
|
