Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Research

Cryoprotective Assessment of Egg Yolk, Low Density Lipoprotein and Their Combination through Antioxidative Enzyme Level in Buck Semen

Mukul Anand, Sarvajeet Yadav, Arun Kumar Madan, Shalini Vaswani, Pankaj Kumar Shukla.

Effect of egg yolk, low density lipoproteins and their combination on live percentage and its correlation with antioxidative enzyme level in Barbari buck spermatozoa was evaluated. Semen was collected biweekly from four healthy Barbari bucks using artificial vagina and pooled. Three aliquots, viz. T-1, T-2 and T-3 were prepared for treatment. T-1 was diluted with extender containing 15% egg yolk, T-2 with extender containing 8% LDL while T-3 with extender containing 15% egg yolk supplemented with 3% LDL to reach final concentration of 200 million spermatozoa per ml. Diluted samples were cryopreserved and evaluated for live percentage and antioxidative enzymes after equilibration and freeze-thawing process. Significantly higher values of antioxidative enzymes viz., Catalase, Superoxide dismutase, Glutathione reductase and Glutathione peroxidase were observed in T-3 as followed by T-2 and T-1, both after equilibration and freeze thawing. A high correlation was observed between live percentage of spermatozoa and Catalase, Superoxide dismutase, Glutathione reductase and Glutathione peroxidase at different stages of cryopreservation and thawing. It can be concluded that the extender containing 15% egg yolk and 3% LDL has better cryoprotective capacity to regulate ROS level as compared to LDL or egg yolk alone for semen cryopreservation.

Key words: Antioxidative enzyme, cryopreservation, lipoprotein, semen, yolk

Full-text options

Full-text Article

Progress in Orthopedic Science


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.