Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Research

Nig. J. Basic Appl. Sci.. 2018; 26(1): 128-131

Effects of Caffeine on the Seed Germination of Bambara Groundnut (Vigna subterranean (L.) Verdc)

Mshelmbula Barka Peter, Titilayo Thomas, Wante Solomon, Okogba John.

Bambara groundnut is an important food legume crop, cultivated mainly in sub-Saharan Africa. Crop plants that are indigenous to the local people are also a valuable biological resource for screening and evaluating the toxicity of the chemical substances with mutagenic effects. In this study, different caffeine concentrations were used to investigate their effect on seed germination of Bambara groundnut. The seeds were pre-soaked in distilled water for 12 hours and then exposed to the different concentrations of the caffeine dose of 0.0, 0.05, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75 and 2.0%, prepared in a phosphate buffer of pH-7. Three replications of the treatments were made and sown each seed of the treatment concentrations in a pot to rise the M1 generation using Complete Randomized Block Design (CRBD). Seed germination was scored for seven days and the germinated seedlings were further observed for 9 weeks. The radicle length, shoot height, and stem girth was measured at the 13 weeks after germination. From the results, it was evident that increased concentration of caffeine reduced the germination percentage of the seeds. In the caffeine concentrations of 1.75-2.0% recorded no germination compare with more than 90% germination at the end of the 7 days of 0.05-0.25% caffeine concentrations. There was a reduction in radicle length as the caffeine concentration increased at 7 weeks after planting. 0.5% caffeine concentration recorded the highest shoot length of 3.32cm among the caffeine concentrations with no significant differences detected in the stem girth studied.

Key words: Key words: Caffeine, Bambara nuts, germination

Share this Article

Oxidants and Antioxidants in Medical Science


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.