Abstract

Aptamers, which are short single-stranded DNA or RNA oligonucleotides, demonstrate remarkable affinity and selectivity for molecular targets, such as cancer biomarkers. Their high stability, structural versatility, and little immunogenicity have generated significant research interest in their diagnostic, therapeutic, and nanomedical applications. We performed integrated bibliometric and thematic analysis of 2,721 research publications indexed in Scopus from 2005 to 2024 to deliver a thorough perspective of this expanding field, utilizing VOSviewer and Bibliometrix to map productivity, collaboration networks, and thematic evolution. China accounted for nearly half of all publications, while the United States led in citation impact per article. Thematic mapping revealed fundamental “basic themes” such as Systematic Evolution of Ligands by Exponential enrichment (SELEX) and nanoparticles, alongside “emerging frontiers” including aptamer-guided drug delivery, AS1411-mediated targeting, exosome-based therapies, and photothermal nanoplatforms. Temporal and citation impact analyses revealed “sleeping beauties” like exosomes and nucleolin that recently gained strong influence, contrasting with earlier “transient hotspots” such as SELEX and biosensors. Enhancing cross-regional cooperation and intercontinental alliances could improve methodological harmonization and expedite the global translation of aptamer technologies. Collectively, this study integrates bibliometric metrics with critical interpretation to provide practical insights for enhancing global collaboration and identifying promising translational pathways in aptamer-based cancer research.

Key words: Aptamers, AS1411, bibliometric, Aptasensor, liposomes, good health and well-being