Abstract

Background:
Propolis resin could be used as a therapeutic agent for chicken embryos, but its toxicity must be evaluated before application in livestock.

Aim:
This study aimed to investigate the toxicity and growth-promoting properties of propolis resin on the embryo of the chicken.

Methods:
Fertilized broiler eggs were used as experimental samples by being injected with propolis resin. The solution volume was predetermined to be 1 ml per egg for each treatment dose.

Results:
The result showed that the morphology of chicken eggs was viable after propolis resin injection on days 13–21 after inoculation. Moreover, 62.5 mg/egg was the effective dose, 125 mg/egg was the Toxic dose 250 mg/egg was the lethal dose. A mid-range dose (62.5–125 mg/egg) was associated with higher embryo weight and wing bone length compared to controls, though differences require further confirmation.

Conclusion:
Propolis resin at intermediate concentration can boost embryonic growth, but higher doses rapidly induce toxicity. These findings emphasize the importance of dose optimization and chemical profiling before in-ovo implementation. Further studies across poultry species are needed to evaluate antiviral and growth-promoting effects while ensuring safety.

Key words: Chicken embryo; Food production; Propolis resin; Toxicity.