Abstract

Staphylococcus aureus is a common micro flora present on skin and mucous membranes of cattle. Despite of it’s the amiable nature; S. aureus can turn shrewd and get to be distinctly pathogenic. Staphylococcus present in the milk may produce numerous potential destructive variables, like toxins. Consequently consumption of S. aureus contaminant milk may results in Staphylococcal food poisoning. Therefore the present study is carried out to evaluate the existence Se-encoding genes in S.aureus and also validate the detection of toxin genes directly from the milk sample contaminated with S. aureus using real-time PCR. The rt-PCR results were reported as Ct values, giving the number of cycle at which the amplification of that particular gene touched the threshold line, which confirmed the detection of enterotoxin genes in the S. aureus isolates and also directly from milk sample. Application of rt-PCR for detection of Se genes in cultures of S. aureus and also from milk samples revealed the probable onset of Staphylococcal food poisoning. Therefore, the study suggests the analysis of milk for the presence of any toxin genes should be strictly performed before reaching the consumer.

Key words: S. aureus; Enterotoxin; Mastitis; Food Poisoning; rt-PCR; Milk