Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research

Fundam Appl Agric. 2019; 4(1): 649-654


Isolation and identification of Clostridium chauvoei from cattle in Mymensingh

Md Nur Alam, Md Muket Mahmud, Jannatun Nime, Roknuzzaman Khan, Md Shahidur Rahman Khan, Md Bahanur Rahman, K H M Nazmul Hussain Nazir.

Abstract
Black quarter (BQ), caused by Clostridium chauvoei, is an economically important, highly infectious bacterial disease of cattle, goat and sheep. The present study was conducted to isolate and identify the causal agent of BQ from field cases. A total of 32 samples from necropsied areas of cattle presented to the Teaching Veterinary Hospital, Bangladesh Agricultural University were collected. The samples were subjected for bacteriological culture followed by identification through a series of conventional bacteriological techniques, staining properties and biochemical tests. The bacteria were cultured in anaerobic condition using candle jar. Morphological characters of bacteria were observed after Gram stain. The suspected samples were subjected for several passages in liver infusion broth to get pure culture. On neomycin blood agar, the bacteria showed hemolysis. Based on the cultural and staining characteristics and biochemical tests, 4 (12.5%) out of 32 samples were confirmed as Cl. chauvoei. It is concluded that Cl. chauvoei has been successfully isolated and identified from BQ affected cattle.

Key words: Black quarter, Clostridium chauvoei, Neomycin blood agar, Liver infusion broth, Cattle



Share this Article


Advertisement
Journal of Complementary Medicine Research

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.