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Original Article

JVS. 2018; 3(1): 68-77
doi: 271758

Identification, characterization and ultrastructure aspects of Alfalfa mosaic virus infecting potato (Solanum tuberosum L.) in Egypt

Maha Ahmed Maher El-Abhar, Moustafa Ahmed Slama Elkady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila.

Aim: The purpose of this study is to characterize biologically and serologically AMV infecting potato (Solanum tuberosum L.) in Egypt. Moreover, the study described the histological and cytological effects of AMV infection in potato leaf cells.
Background: Alfalfa mosaic virus (AMV) is only virus in the genus Alfamovirus and has very wide host range among weed and crop plants which produces a variety of symptoms. It can cause problems in potato in some regions where vectors easily move into potato fields from reservoir host, particularly if a tuber necrosis-causing strain is involved.
Methods: Leaf samples were collected on the basis of visual symptoms from potato plants with yellow blotching symptoms, called “Calico” and leaf distortion. A sap-transmitted virus isolated from potato was biologically purified after three successive single local lesion passages onto Chenopodium ammaranticolor which reproduced prominent local lesions. The virus isolate was then propagated in potato Ditta cv. plants, The virus was identified on the bases of host range, symptomatology, transmission and serological diagnosis, in addition to the ultrastructural changes pro
duced in potato leaf cells infected with AMV
Results: Reaction of thirteen plant species and cultivars belonging to four families (Amaranthaceae, Solanaceae, Fabaceae and Laminaceae) to AMV infection was demonstrated. The presence or absence of the virus was verified by back inoculation onto healthy indicator host plant and/or ELISA test. AMV was readily transmitted by mechanical means and by Myzus persicae with percentage of 60 %. In addition to visible symptoms, infection with AMV also causes ultrastructural changes in potato leaf cells. Examination of epidermal strips of N. tabacum cv. White Burley using light microscope showed amorphous cytoplasmic inclusion bodies seemed to be attached to the nucleus from one or two sites, while those inclusions have never been observed in the epidermal stripes of healthy leaves. Electron microscopy, revealed cytological and histological changes induced by Alfalfa mosaic virus infection in potato leaves.
Conclusion: In this work, Alfalfa mosaic virus (AMV) characterized and ultrastructure aspected of infecting potato (Solanum tuberosum L. ) in Egypt illustrating important effect of AMV on potato plant

Key words: Key words: Potato (Solanum tuberosum), Alfalfa mosaic virus, Host range, Transmission, ELISA, Electronmicroscope and Ultrastructural changes

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