Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

JVS. 2018; 3(1): 68-77
doi: 271758


Identification, characterization and ultrastructure aspects of Alfalfa mosaic virus infecting potato (Solanum tuberosum L.) in Egypt

Maha Ahmed Maher El-Abhar, Moustafa Ahmed Slama Elkady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila.

Abstract
ABSTRACT
Aim: The purpose of this study is to characterize biologically and serologically AMV infecting potato (Solanum tuberosum L.) in Egypt. Moreover, the study described the histological and cytological effects of AMV infection in potato leaf cells.
Background: Alfalfa mosaic virus (AMV) is only virus in the genus Alfamovirus and has very wide host range among weed and crop plants which produces a variety of symptoms. It can cause problems in potato in some regions where vectors easily move into potato fields from reservoir host, particularly if a tuber necrosis-causing strain is involved.
Methods: Leaf samples were collected on the basis of visual symptoms from potato plants with yellow blotching symptoms, called “Calico” and leaf distortion. A sap-transmitted virus isolated from potato was biologically purified after three successive single local lesion passages onto Chenopodium ammaranticolor which reproduced prominent local lesions. The virus isolate was then propagated in potato Ditta cv. plants, The virus was identified on the bases of host range, symptomatology, transmission and serological diagnosis, in addition to the ultrastructural changes pro
duced in potato leaf cells infected with AMV
Results: Reaction of thirteen plant species and cultivars belonging to four families (Amaranthaceae, Solanaceae, Fabaceae and Laminaceae) to AMV infection was demonstrated. The presence or absence of the virus was verified by back inoculation onto healthy indicator host plant and/or ELISA test. AMV was readily transmitted by mechanical means and by Myzus persicae with percentage of 60 %. In addition to visible symptoms, infection with AMV also causes ultrastructural changes in potato leaf cells. Examination of epidermal strips of N. tabacum cv. White Burley using light microscope showed amorphous cytoplasmic inclusion bodies seemed to be attached to the nucleus from one or two sites, while those inclusions have never been observed in the epidermal stripes of healthy leaves. Electron microscopy, revealed cytological and histological changes induced by Alfalfa mosaic virus infection in potato leaves.
Conclusion: In this work, Alfalfa mosaic virus (AMV) characterized and ultrastructure aspected of infecting potato (Solanum tuberosum L. ) in Egypt illustrating important effect of AMV on potato plant

Key words: Key words: Potato (Solanum tuberosum), Alfalfa mosaic virus, Host range, Transmission, ELISA, Electronmicroscope and Ultrastructural changes



Similar Articles

Passive social media use and psychological well-being during the COVID-19 pandemic: The role of social comparison and emotion regulation.
Yue Z, Zhang R, Xiao J
Computers in human behavior. 2022; 127(): 107050

Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 209-220

Using the Health Belief Model to examine travelers' willingness to vaccinate and support for vaccination requirements prior to travel.
Suess C, Maddock J, Dogru T, Mody M, Lee S
Tourism management. 2022; 88(): 104405

Exploring the Alzheimer's disease neuroepigenome: recent advances and future trends.
Zhang H, Elefant F
Neural regeneration research. 2022; 17(2): 325-327

Growth differentiation factor 5: a neurotrophic factor with neuroprotective potential in Parkinson's disease.
Goulding SR, Anantha J, Collins LM, Sullivan AM, O'Keeffe GW
Neural regeneration research. 2022; 17(1): 38-44


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.



ScopeMed Web Sites