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Original Article



Location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi carried by pea seeds

ASHWANI KUMAR VERMA, KAILASH AGRAWAL.




Abstract
Cited by 4 Articles

The present study aims to determine the location and histopathology of seed borne bacterial pathogen Paeudomonas syringae pv. pisi associated with pea seeds. The pea seeds categorized (Asymptomatic, Moderately discoloured and Shrivelled discoloured seeds) and seeds of each category were assayed for the presence of bacterial pathogen P. s. pv. pisi. Seed samples carrying high incidence (89.5% and 91.34%) of bacterial pathogen were used for the microtome sectioning individually. It was observed that the cells of P. syringae pv. pisi were found in large numbers into radicle and hilum region and into space between seed coat and spermoderm, in shrivelled discoloured seed category while in moderately discoloured seed category bacterial cells were less in numbers and found into inner side of seed coat and endosperm. Bacterial colonisation within seed tissue caused necrosis, formation of lytic cavities and reduction in cell contents.

Key words: Categorization, Incidence, Pea Seeds, P. syringae pv. pisi, Symptoms.






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