A study was carried out to assess the effect of semen collection interval and semen characteristics of ostriches aged four to nine years for a period of 12 months in tropical climate of India. The birds were grouped into two categories viz., four to six and seven to nine years-old. The artificial cloaca was used for semen collection. The ostriches were allowed to mate naturally by following the teaser technique. The overall mean values for semen collection interval, semen volume, pH, mass activity (score), spermatozoa motility, concentration, live and abnormal spermatozoa of ostrich were 17.36 days, 1.38 ml, 7.28, 4.26, 79.21 % , 2.69 ´ 109 per ml, 85.71 % and 15.30 % respectively. The individual males showed varied response and significant (P≤0.01) differences in all seminal attributes. The effect of age, month and season had significant (P≤0.01) influence on seminal attributes. The mean semen collection interval and semen characteristics in four to six year-old ostrich were better than seven to nine year-old ostrich. Overall, the vital seminal attributes like spermatozoa motility, concentration, live and abnormal spermatozoa were better during winter and summer than southwest and northeast monsoon seasons.
Key words: Semen Collection Intervals, Semen Characteristics, Ostrich