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Cryopeservation Induced Alteration in Sperm Morphology in Hariana Bull Semen

Chetna Gangwar, Atul Saxena, Pankaj Kumar Shukla, Shri Prakash Singh, Akhil Patel, Mohit Antil, Abhinov Verma.

Abstract
Present study was conducted to evaluate the effect of semen cryopreservation on sperm morphology. For this 4 Hariana bulls were selected and 8 semen ejaculates from each bull were collected through artificial vagina method at twice a week interval. After preliminary semen evaluation, suitable semen samples were diluted with glycerolated egg yolk TRIS diluter. Semen samples were cryopreserved following the equilibration of 4h at 5˚C and finally stored into liquid nitrogen container. For sperm morphology head length, head width, base width, head area, mid piece length, tail length, total length, head ellipticity, head elongation and head regularity was measured in fresh as well as cryopreserved semen samples. Findings of the study revealed that there is a significant (P< 0.05) reduction in head area and other measurements after cryopreservation. The study was concluded with that cryopreservation induces various morphological changes in sperm which might be responsible for reduced fertility of frozen thawed spermatozoa.

Key words: Cryopeservation, Fertility, Hariana Bull, Sperm Morphometry



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