Home|Journals|Articles by Year|Audio Abstracts

Original Article

J App Pharm Sci. 2017; 7(10): 118-124

Antihypertensive and Vasorelaxant effects of the ethanolic extract from the stem bark of Aspidosperma tomentosum Mart

Fabíola Fialho Furtado, Camilla Pinheiro de Menezes, Vanderson Barbosa Bernardo, Antônio Euzébio Goulart Santana, Marcos José Salvador, João Xavier de Araújo-Júnior, Emanuel Tenório Paulino, Jessyka Carolina Galvão da Silva, Êurica Adélia Nogueira Ribeiro, Isac Almeida de Medeiros.


Aspidosperma species have been traditionally used for the treatment of cardiovascular disease. These species are important sources of indole alkaloids, which are responsible for different cardiovascular effects. Based on this premise, and as there are still no studies related to the cardiovascular effects of the ethanolic extract from the stem bark of Aspidosperma tomentosum (EEAT). The aim of the present study was to evaluate the possible mechanism of the vasorelaxant action of EEAT in isolated rat mesenteric rings, and to establish its antihypertensive activity. The mean arterial pressure (MAP) and Heart Rate (HR) were measured in hypertensive rats through a catheter inserted in abdominal aorta via femoral artery. The vasodilator effect of EEAT in isolated pre-contracted rat mesenteric rings was examined. The bioactive extract was analyzed via mass spectrometry fingerprinting. EEAT induced relaxation in mesenteric rings pre-contracted with Phe independent of the endothelium. In denuded rings, when incubation with KCl 20 mM or TEA did not change the relaxation. EEAT inhibited the concentration-response curves induced by CaCl2 or Phe and inhibited 80 mM KCl-induced contraction. In pre-contracted preparations with BayK 8644, the EEAT induced relaxation. The transient contraction induced by Phe was inhibited. The oral administration of EEAT reduced MAP and did not alter the HR. Phenolic acids, flavonoids and indole alkaloids were identified in EEAT. In conclusions, the EEAT induced vasorelaxation through a blockade of Ca2+ channels and inhibition of the mobilization of Ca2+ of the IP3-sensivite Ca2+ stores and caused an antihypertensive effect.

Key words: Aspidosperma tomentosum, vasorelaxant activity, mesenteric rings, Ca2+ influx, antihypertensive effect, rats

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.