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Original Article

J App Pharm Sci. 2017; 7(9): 207-212


Chemical Composition and Antimicrobial Activity of the Essential Oil of the Leaves of Cupressus macrocarpa Hartweg. ex Gordon

Amal M. Saad, Magdy M.D. Mohammed, Mosad A. Ghareeb, Wafaa S. Ahmed, Mohamed A. Farid.




Abstract

Qualitative (GC-MS) and quantitative (GC-FID) analyses of the essential oil (EO) isolated from the leaves of Cupressus macrocarpa revealed the presence of fifteen components, and representing 86.29% of the total oil composition. Their identification were based on their retention times and mass spectral fragmentation patterns. α-terpineol (19.01%), camphenilone (9.78%), elemol (8.92%), 2-tridecanone (8.75%), α-terpinyl acetate (8.62%), 2-pentyl-2-cyclopenten-1-one (6.90%) and β-bisabolol (5.83%) represent major components. Agar diffusion method was conducted to test the antimicrobial activity of the EO against eight pathogenic strains, including gram (+ve) and (-ve) bacteria and fungi. The obtained results showed inhibition zones with 10.5-21mm diameters, compared to Streptomycin.

Key words: Monterey Cypress, Cupressaceae, Volatile constituents, GC/MS, GC/FID, Antibacterial activity, Antifungal activity, MIC






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