Cytotoxic activity of twenty extracts of fungal derived from marine sponge Haliclona fascigera collected from West Sumatera was evaluated by using Brine Shrimps Lethality Test (BSLT) and MTT assay on Hela, WiDr, T47D and Vero cells. In BSLT result, all fungal extracts were cytotoxic because their LC50 < 1000 ppm, and ranging from 1 to 335 ppm. While sixteen extracts (80%) were toxic (LC50 100 ppm) and further tested its cytotoxic activity against HeLa, Widr, T47D and Vero cell lines. A single 100 µg/mL dose of each extract was employed with 72 h of incubation for all tests. Doxorubicin was used as the positive control. The fungal extract of WR6 showed the highest MTT cytotoxicity with IC50 values of 47.4, 67.1, 118.3 and 163.37 ppm against WiDr, T47D, HeLa and Vero cell lines respectively. Therefore, this extract was chosen to be a good candidate for further studies as a new anticancer drug in the treatment of human cancer.
Key words: Cytotoxic activity, Haliclona fascigera, marine derived fungi, Brine Shrimp Lethality Test (BSLT), MTT assay.
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