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Original Research

Occurrence of Bovine and Human Methicillin-Resistant Staphylococcus aureus in Organised Dairy Farms of Andhra Pradesh, India

Shaik Reshma, Tumati Srinivasa Rao, Thirtham Madhava Rao, Kothapalli Venkata Subramanyam, Madupuru Soma Sekhar.


The present study was undertaken for isolation and molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) from nasal swabs of apparently healthy cattle, buffaloes and their handlers. Out of 513 nasal swab samples analyzed, 162 (31.6%) S. aureus isolates were recovered, of which 104 (20.2%) were found positive for coagulase enzyme production. On MeReSa HICHROM agar supplemented with cefoxitin, bluish green colonies were observed in 100% coagulase positive S. aureus isolates of livestock handlers followed by buffaloes (48.6%) and cattle (24%). Disc diffusion test showed resistance to both oxacillin and cefoxitin in 7 (77.7%), 16 (43.7%) and 20 (33.3%) coagulase positive isolates of livestock handlers, buffaloes and cattle, respectively. Both mecA and blaZ genes were detected in 3 (33.3%), 10 (28.6%) and 11 (18.6%) coagulase positive isolates of livestock handlers, buffaloes and cattle, respectively. Correlation between phenotypic resistance to oxacillin, cefoxitin and presence of mecA gene was not observed.

Key words: MRSA, Coagulase, PCR, mecA, blaZ

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